NDP52 Recombinant Rabbit Monoclonal Antibody [PSH02-33]
cat.: HA721356
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH02-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 52 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human NDP52 aa 1-150 / 446 (Q13137). |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, Raji cell lysate, THP-1 cell lysate, Huh7 cell lysate, HepG2 cell lysate, Saos-2 cell lysate, RAW264.7 cell lysate, HeLa. |
| Subcellular location: | Cytoplasm, perinuclear region, cytoskeleton, cytoplasmic vesicle, autophagosome membrane |
| Recommended Dilutions:
WB FC |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q13137 human |
| Alternative names: | Antigen nuclear dot 52 kDa protein CACO2_HUMAN Calcium binding and coiled coil domain 2 Calcium binding and coiled coil domain containing protein 2 Calcium-binding and coiled-coil domain-containing protein 2 CALCOCO 2 CALCOCO2 MGC17318 NDP52 Nuclear domain 10 protein 52 Nuclear domain 10 protein Nuclear domain 10 protein NDP52 Nuclear domain protein 52 Nuclear dot protein 52 OTTHUMP00000218344 OTTHUMP00000218345 OTTHUMP00000218346 OTTHUMP00000218347 OTTHUMP00000218349 OTTHUMP00000218351 |
Images
|
Fig1:
Western blot analysis of NDP52 on different lysates with Rabbit anti-NDP52 antibody (HA721356) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: Raji cell lysate Lane 4: THP-1 cell lysate Lane 5: Huh7 cell lysate Lane 6: HepG2 cell lysate Lane 7: Saos-2 cell lysate Lane 8: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721356) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HeLa cells labeling NDP52. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721356, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.