4-1BBL Recombinant Rabbit Monoclonal Antibody [JE33-91]
cat.: HA721357
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE33-91 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human 4-1BBL aa 205-254 / 254. |
| Positive control: | THP-1 whole cell lysate, THP-1 treated with 100nM TPA overnight whole cell lysate, HeLa cell lysate, HepG2 cell lysate, RAW264.7 cell lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P41273 Human | P41274 Mouse |
| Alternative names: | 4 1BB L 4 1BB ligand 4 1BBL 4-1BB ligand 4-1BBL Cd137l Cd157l Homolog of mouse 4 1BB L Homolog of mouse 4 1BBL ILA ligand (TNF related) Ly63l Receptor 4 1BB ligand TNF superfamily member 9 TNFL9_HUMAN Tnfsf9 TNLG5A Tumor necrosis factor (ligand) superfamily member 9 Tumor necrosis factor ligand 5A Tumor necrosis factor ligand superfamily member 9 Tumor necrosis factor superfamily member 9 |
Images
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Fig1:
Western blot analysis of 4-1BBL on different lysates with Rabbit anti-4-1BBL antibody (HA721357) at 1/1,000 dilution. Lane 1: THP-1 whole cell lysate Lane 2: THP-1 treated with 100nM TPA overnight whole cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 27 kDa Observed band size: 29 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721357) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.