CCR8 Recombinant Rabbit Monoclonal Antibody [JE31-99]
cat.: HA721360
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE31-99 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CCR8 aa 281-355 / 355. |
| Positive control: | Mouse thymus tissue lysates, rat thymus tissue lysates, Raji, PC-3. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:50 |
| Uniprot #: | SwissProt: P51685 Human | P56484 Mouse Entrez Gene: 301066 Rat |
| Alternative names: | C C chemokine receptor type 8 C C CKR 8 C C motif chemokine receptor 8 C-C chemokine receptor type 8 C-C CKR-8 CC chemokine receptor 8 CC chemokine receptor CHEMR1 CC chemokine receptor type 8 CC CKR 8 CC-CKR-8 CCR 8 CCR-8 Ccr8 CCR8 protein CCR8-L CCR8_HUMAN CDw198 CDw198 antigen Chemokine (C C motif) receptor 8 Chemokine (C C) receptor 8 Chemokine (C C) receptor like 2 Chemokine (CC motif) receptor 8 Chemokine (CC) receptor 8 Chemokine (CC) receptor like 1 Chemokine (CC) receptor like 2 Chemokine C C motif receptor 8 Chemokine C C receptor 8 Chemokine CC motif receptor 8 Chemokine CC receptor 8 Chemokine receptor 8 Chemokine receptor like 1 Chemokine receptor-like 1 CKR L1 CKR-L1 CKRL 1 CKRL1 CMKBR 8 CMKBR L2 CMKBR8 CMKBRL 2 CMKBRL2 CY 6 CY6 GPR CY6 GPR-CY6 GPRCY6 MGC123958 MGC123959 MGC129966 MGC129973 TER 1 TER1 |
Images
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Fig1:
Western blot analysis of CCR8 on mouse thymus tissue lysates with Rabbit anti-CCR8 antibody (HA721360) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 41 kDa Observed band size: 48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721360) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of CCR8 on rat thymus tissue lysates with Rabbit anti-CCR8 antibody (HA721360) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 41 kDa Observed band size: 48 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721360) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of Raji cells labeling CCR8 with Rabbit anti-CCR8 antibody (HA721360) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CCR8 antibody (HA721360) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig4:
Immunocytochemistry analysis of PC-3 cells labeling CCR8 with Rabbit anti-CCR8 antibody (HA721360) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CCR8 antibody (HA721360) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.