MYL9 Recombinant Rabbit Monoclonal Antibody [JE34-63]
cat.: HA721361
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE34-63 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MYL9 aa 51-100 / 172. |
| Positive control: | Hela cell lysate, mouse colon tissue lysate, rat colon tissue lysate, rat uterus tissue lysate, human colon tissue, human skin tissue, mouse heart tissue, rat skeletal muscle tissue, human stomach tissue lysates. |
| Subcellular location: | Cytoplasm, cytoskeleton, cell cortex. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P24844 Human | Q9CQ19 Mouse | Q64122 Rat |
| Alternative names: | 20 kDa myosin light chain Human 20kDa myosin light chain (MLC2) mRNA complete cds LC20 MGC3505 MLC 2 MLC-2C MLC2 MLY 9 MRLC1 MYL9 MYL9_HUMAN Myosin light chain 9 regulatory Myosin light polypeptide 9 regulatory myosin regulatory light chain 1 Myosin regulatory light chain 2 Myosin regulatory light chain 2 smooth muscle isoform Myosin regulatory light chain 9 Myosin regulatory light chain MRLC1 Myosin regulatory light polypeptide 9 Myosin RLC Myosin vascular smooth muscle light chain 2 MYRL2 OTTHUMP00000030857 smooth muscle isoform |
Images
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Fig1:
Western blot analysis of MYL9 on different lysates with Rabbit anti-MYL9 antibody (HA721361) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: Mouse colon tissue lysate Lane 3: Rat colon tissue lysate Lane 4: Rat uterus tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 20 kDa Observed band size: 19 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721361) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of MYL9 on human stomach tissue lysates with Rabbit anti-MYL9 antibody (HA721361) at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Predicted band size: 20 kDa Observed band size: 19 kDa Exposure time: 1 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721361) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-MYL9 antibody (HA721361) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721361) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-MYL9 antibody (HA721361) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721361) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse heart tissue with Rabbit anti-MYL9 antibody (HA721361) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721361) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue with Rabbit anti-MYL9 antibody (HA721361) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721361) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.