E2F4 Recombinant Rabbit Monoclonal Antibody [PSH0-45]
cat.: HA721362
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH0-45 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human E2F4 aa 21-80 / 413. |
| Positive control: | 293T cell lysate, Raji cell lysate, HeLa cell lysate, Jurkat cell lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q16254 Human |
| Alternative names: | E2F 4 E2F transcription factor 4 E2F transcription factor 4 p107/p130 binding E2F-4 E2F4 E2F4_HUMAN p107/p130 binding protein Transcription factor E2F 4 Transcription factor E2F4 |
Images
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Fig1:
Western blot analysis of E2F4 on different lysates with Rabbit anti-E2F4 antibody (HA721362) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Raji cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 10 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721362) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.