AKT1 Recombinant Rabbit Monoclonal Antibody [PSH0-50]
cat.: HA721364
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | PSH0-50 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55.7 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human AKT1 aa 271-320 / 480. |
| Positive control: | Jurkat cell lysate, MCF7 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, COS-1 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, Jurkat. |
| Subcellular location: | Cytoplasm, Nucleus, Cell membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:50 1:50-1:1,000 |
| Uniprot #: | SwissProt: P31749 Human | P31750 Mouse | P47196 Rat |
| Alternative names: | AKT 1 AKT AKT1 AKT1_HUMAN C AKT cAKT MGC99656 PKB alpha PKB PKB-ALPHA PRKBA Protein Kinase B Alpha Protein kinase B Proto-oncogene c-Akt RAC Alpha RAC Rac protein kinase alpha RAC Serine/Threonine Protein Kinase RAC-alpha serine/threonine-protein kinase RAC-PK-alpha v akt murine thymoma viral oncogene homolog 1 vAKT Murine Thymoma Viral Oncogene Homolog 1 |
Images
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Fig1:
Western blot analysis of AKT1 on different lysates with Rabbit anti-AKT1 antibody (HA721364) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: MCF7 cell lysate Lane 3: HeLa cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: C2C12 cell lysate Lane 6: COS-1 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 56 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721364) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of Jurkat cells labeling AKT1 with Rabbit anti-AKT1 antibody (HA721364) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-AKT1 antibody (HA721364) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig3:
Flow cytometric analysis of Jurkat cells labeling AKT1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721364, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.