LFNG Recombinant Rabbit Monoclonal Antibody [JE66-62]
cat.: HA721367
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE66-62 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 42 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human LFNG aa 330-379 / 379. |
| Positive control: | Hela cell lysate, K-562 cell lysate, HL-60 cell lysate, MCF7 cell lysate, HT-29 cell lysate, C2C12 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, HUVEC, MCF7, K-562. |
| Subcellular location: | Golgi apparatus membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:500 1:1,000 |
| Uniprot #: | SwissProt: Q8NES3 Human | O09010 Mouse |
| Alternative names: | Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase |
Images
|
Fig1:
Western blot analysis of LFNG on different lysates with Rabbit anti-LFNG antibody (HA721367) at 1/1,000 dilution. Lane 1: Hela cell lysate (15 µg/Lane) Lane 2: K-562 cell lysate (15 µg/Lane) Lane 3: HL-60 cell lysate (15 µg/Lane) Lane 4: MCF7 cell lysate (15 µg/Lane) Lane 5: HT-29 cell lysate (15 µg/Lane) Lane 6: C2C12 cell lysate (15 µg/Lane) Lane 7: PC-12 cell lysate (15 µg/Lane) Lane 8: Mouse testis tissue lysate (30 µg/Lane) Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 7 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721367) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunocytochemistry analysis of HUVEC cells labeling LFNG with Rabbit anti-LFNG antibody (HA721367) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LFNG antibody (HA721367) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig3:
Immunocytochemistry analysis of MCF7 cells labeling LFNG with Rabbit anti-LFNG antibody (HA721367) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LFNG antibody (HA721367) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
|
Fig4:
Flow cytometric analysis of K-562 cells labeling LFNG. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721367, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.