GPCR LGR6 Recombinant Rabbit Monoclonal Antibody [JE39-78]
cat.: HA721370
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell
Clonality: Monoclonal
Clone number: JE39-78
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 104 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human GPCR LGR6 aa 831-967 / 967.
Positive control: HeLa cell lysate, MCF7 cell lysate, SKOV-3 cell lysate, mouse testis tissue lysate, rat testis tissue lysates, human lung carcinoma tissue, human testis tissue, mouse kidney tissue, mouse liver tissue, HeLa.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
1:1,000
1:1,000
1:100
Uniprot #: SwissProt: Q9HBX8 Human | Q3UVD5 Mouse
Entrez Gene: 498233 Rat
Alternative names: FLJ14471 Gonadotropin receptor GPCR Leucine rich repeat containing G protein coupled receptor 6 Leucine-rich repeat-containing G-protein coupled receptor 6 Lgr6 LGR6_HUMAN VTS20631
Images
HA721370_1.jpg Fig1: Western blot analysis of GPCR LGR6 on different lysates with Rabbit anti-GPCR LGR6 antibody (HA721370) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (10 µg/Lane)
Lane 2: MCF7 cell lysate (10 µg/Lane)
Lane 3: SKOV-3 cell lysate (10 µg/Lane)
Lane 4: Mouse testis tissue lysate (20 µg/Lane)

Predicted band size: 104 kDa
Observed band size: 104 kDa

Exposure time: 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721370) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721370_2.jpg Fig2: Western blot analysis of GPCR LGR6 on rat testis tissue lysates with Rabbit anti-GPCR LGR6 antibody (HA721370) at 1/1,000 dilution.

Lysates/proteins at 20 µg/Lane.

Predicted band size: 104 kDa
Observed band size: 104 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721370) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721370_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-GPCR LGR6 antibody (HA721370) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721370_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-GPCR LGR6 antibody (HA721370) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721370_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-GPCR LGR6 antibody (HA721370) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721370_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-GPCR LGR6 antibody (HA721370) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721370) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721370_7.jpg Fig7: Immunocytochemistry analysis of HeLa cells labeling GPCR LGR6 with Rabbit anti-GPCR LGR6 antibody (HA721370) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-GPCR LGR6 antibody (HA721370) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.