CD48 Recombinant Rabbit Monoclonal Antibody [JE37-98]
cat.: HA721371
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE37-98 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27.7 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human CD48 aa 144-243 / 243. |
| Positive control: | Raji cell lysate, Jurkat cell lysate, human colon tissue, human kidney tissue. |
| Subcellular location: | Cell membrane, Secreted |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P09326 Human | P18181 Mouse | P10252 Rat |
| Alternative names: | Antigen CD48 B cell membrane protein B lymphocyte activation marker BLAST 1 B-cell activation marker B-lymphocyte activation marker BLAST-1 BCM 1 surface antigen BCM1 BCM1 surface antigen BLAST 1 BLAST BLAST1 CD 48 CD48 CD48 antigen (B cell membrane protein) CD48 antigen CD48 molecule CD48 protein CD48_HUMAN hCD48 Leucocyte antigen MEM 102 Leukocyte antigen MEM-102 mCD48 MEM 102 MEM-102 MEM102 Signaling lymphocytic activation molecule 2 SLAM family member 2 SLAMF 2 SLAMF2 TCT.1 |
Images
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Fig1:
Western blot analysis of CD48 on different lysates with Rabbit anti-CD48 antibody (HA721371) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 27.7 kDa Observed band size: 40 kDa Exposure time: 80 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721371) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-CD48 antibody (HA721371) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD48 antibody (HA721371) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.