TR4 Recombinant Rabbit Monoclonal Antibody [JE45-03]
cat.: HA721372
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE45-03 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 65 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TR4 aa 497-596 / 596. |
| Positive control: | Hela cell lysate, SH-SY5Y cell lysate, Jurkat cell lysate, PC-3M cell lysate, NIH/3T3 cell lysate, human pancreas tissue, mouse pancreas tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: P49116 Human | P49117 Mouse | P55094 Rat |
| Alternative names: | hTAK1 Nr2c2 NR2C2_HUMAN Nuclear hormone receptor TR4 Nuclear receptor subfamily 2 group C member 2 Orphan nuclear receptor TAK1 Orphan nuclear receptor TR4 TAK1 Testicular nuclear receptor 4 Testicular receptor 4 TR2R1 TR4 TR4 nuclear hormone receptor |
Images
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Fig1:
Western blot analysis of TR4 on different lysates with Rabbit anti-TR4 antibody (HA721372) at 1/1,000 dilution. Lane 1: Hela cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: Jurkat cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 65 kDa Observed band size: 65 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721372) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-TR4 antibody (HA721372) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721372) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue with Rabbit anti-TR4 antibody (HA721372) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721372) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.