eIF3A Recombinant Rabbit Monoclonal Antibody [JE58-60]
cat.: HA721375
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE58-60 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 167 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human eIF3A aa 275-324 / 1,382. |
| Positive control: | 293T cell lysate, A431 cell lysate, Jurkat cell lysate, Raji cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, L6 cell lysate, PC-12 cell lysate, mouse brain tissue, rat brain tissue, Hela. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:1,000 1:100 |
| Uniprot #: | SwissProt: Q14152 Human | P23116 Mouse | Q1JU68 Rat |
| Alternative names: | centrosomin homolog cytoplasmic protein p167 eIF 3 theta eIF-3-theta EIF3 eIF3 p167 eIF3 p170 eIF3 p180 EIF3 p180 subunit eIF3 p185 eIF3 theta eIF3a EIF3A_HUMAN EIF3S10 eukaryotic translation initiation factor 3 subunit 10 (theta 150 170kD) eukaryotic translation initiation factor 3 subunit 10 (theta 170kD) eukaryotic translation initiation factor 3 subunit 10 170kDa Eukaryotic translation initiation factor 3 subunit 10 eukaryotic translation initiation factor 3 subunit 10 theta 150 170kDa Eukaryotic translation initiation factor 3 subunit A OTTHUMP00000020589 P167 p180 p185 TIF32 |
Images
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Fig1:
Western blot analysis of eIF3A on different lysates with Rabbit anti-eIF3A antibody (HA721375) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 3: Jurkat cell lysate Lane 4: Raji cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: L6 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 167 kDa Observed band size: 167 kDa Exposure time: 7seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721375) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-eIF3A antibody (HA721375) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721375) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-eIF3A antibody (HA721375) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721375) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of Hela cells labeling eIF3A with Rabbit anti-eIF3A antibody (HA721375) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-eIF3A antibody (HA721375) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Immunocytochemistry analysis of A431 cells labeling eIF3A with Rabbit anti-eIF3A antibody (HA721375) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-eIF3A antibody (HA721375) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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