MC1-R Recombinant Rabbit Monoclonal Antibody [JE39-49]
cat.: HA721377
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE39-49 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 35 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human MC1-R aa 1-50 / 317. |
| Positive control: | HepG2 cell lysate, Raji cell lysate, SW620 cell lysate, rat skin tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1: 1,000 |
| Uniprot #: | SwissProt: Q01726 Human | B6ZGR5 Rat |
| Alternative names: | CMM5 MC1 Receptor MC1-R MC1R Melanocortin 1 receptor Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) Melanocortin receptor 1 Melanocyte-stimulating hormone receptor Melanotropin receptor MSH-R MSHR MSHR_HUMAN SHEP2 |
Images
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Fig1:
Western blot analysis of MC1-R on different lysates with Rabbit anti-MC1-R antibody (HA721377) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: Raji cell lysate (15 µg/Lane) Lane 3: SW620 cell lysate (15 µg/Lane) Lane 4: Rat skin tissue lysate (30 µg/Lane) Predicted band size: 35 kDa Observed band size: 35 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721377) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.