c-Maf Recombinant Rabbit Monoclonal Antibody [JE39-57]
cat.: HA721378
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE39-57 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 38 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human c-Maf aa 324-373 / 373. |
| Positive control: | A549 cell lysate, Raji cell lysate, mouse lung tissue lysate, mouse kidney tissue lysate, rat spleen tissue lysate, rat kidney tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O75444 Human | P54843 Mouse | P54844 Rat |
| Alternative names: | AS42 oncogene homolog Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene Avian musculoaponeurotic fibrosarcoma (v maf) c maf proto oncogene cMaf maf MAF_HUMAN MAF2 MGC71685 Proto oncogene c Maf Proto-oncogene c-maf Transcription factor Maf v maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) v maf musculoaponeurotic fibrosarcoma oncogene homolog V-maf musculoaponeurotic fibrosarcoma oncogene homolog |
Images
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Fig1:
Western blot analysis of c-Maf on different lysates with Rabbit anti-c-Maf antibody (HA721378) at 1/1,000 dilution. Lane 1: A549 cell lysate (10 µg/Lane) Lane 2: Raji cell lysate (10 µg/Lane) Lane 3: Mouse lung tissue lysate (20 µg/Lane) Lane 4: Mouse kidney tissue lysate (20 µg/Lane) Lane 5: Rat spleen tissue lysate (20 µg/Lane) Lane 6: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721378) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.