NR5A2 Recombinant Rabbit Monoclonal Antibody [JE30-50]
cat.: HA721379
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE30-50 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human NR5A2 aa 442-541 / 541. |
| Positive control: | MCF7 cell lysate, 293T cell lysate, HepG2 cell lysate, A549 cell lysate, PANC-1 cell lysate, SW1990 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse pancreas tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O00482 Human | P45448 Mouse | Q9QWM1 Rat |
| Alternative names: | Alpha-1-fetoprotein transcription factor B1-binding factor B1F B1F2 CPF CYP7A promoter-binding factor FTF FTZ F1 FTZ F1beta FTZ-F1 FTZ-F1beta hB1F 2 hB1F hB1F-2 Hepatocytic transcription factor Liver receptor homolog 1 LRH-1 LRH1 Nr5a2 NR5A2_HUMAN Nuclear receptor subfamily 5 group A member 2 |
Images
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Fig1:
Western blot analysis of NR5A2 on different lysates with Rabbit anti-NR5A2 antibody (HA721379) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 µg/Lane) Lane 2: 293T cell lysate (15 µg/Lane) Lane 3: HepG2 cell lysate (15 µg/Lane) Lane 4: A549 cell lysate (15 µg/Lane) Lane 5: PANC-1 cell lysate (15 µg/Lane) Lane 6: SW1990 cell lysate (15 µg/Lane) Lane 7: Mouse liver tissue lysate (30 µg/Lane) Lane 8: Rat liver tissue lysate (30 µg/Lane) Lane 9: Mouse brain tissue lysate (30 µg/Lane) Lane 10: Rat brain tissue lysate (30 µg/Lane) Lane 11: Mouse pancreas tissue lysate (30 µg/Lane) Predicted band size: 61 kDa Observed band size: 65 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721379) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.