HA721380

PTGER2 Recombinant Rabbit Monoclonal Antibody [JE30-72]

cat.: HA721380

Product Type:	Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, FC, IF-Cell
Clonality:	Monoclonal
Clone number:	JE30-72

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Protein A affinity purified.
Molecular weight:	Predicted band size: 40 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human PTGER2 aa 300-358 / 358.
Positive control:	JAR cell lysate, NIH/3T3 cell lysate, human placenta tissue lysate, mouse placenta tissue lysate, mouse lung tissue lysate, rat lung tissue lysate, HeLa cell lysate, A549 cell lysate, THP-1 cell lysate, human placenta tissue, human lung tissue, JAR.
Subcellular location:	Cell membrane.
Recommended Dilutions: WB IHC-P FC IF-Cell	1:1,000 1:200-1:1,000 1:500-1:1,000 1:100
Uniprot #:	SwissProt: P43116 Human \| Q62053 Mouse \| Q62928 Rat
Alternative names:	EP2 PE2R2_HUMAN PGE receptor EP2 subtype PGE2 receptor EP2 subtype Prostaglandin E receptor 2 EP2 subtype Prostaglandin E receptor 2 subtype EP2 53kDa Prostaglandin E receptor 2 subtype EP2 Prostaglandin E2 receptor Prostaglandin E2 receptor EP2 subtype Prostanoid EP2 receptor Ptger2

Images

Fig1: Western blot analysis of PTGER2 on different lysates with Rabbit anti-PTGER2 antibody (HA721380) at 1/1,000 dilution.

Lane 1: JAR cell lysate (15 µg/Lane)
Lane 2: NIH/3T3 cell lysate (15 µg/Lane)
Lane 3: Human placenta tissue lysate (30 µg/Lane)
Lane 4: Mouse placenta tissue lysate (30 µg/Lane)
Lane 5: Mouse lung tissue lysate (30 µg/Lane)
Lane 6: Rat lung tissue lysate (30 µg/Lane)

Predicted band size: 40 kDa
Observed band size: 50 kDa

Exposure time: 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721380) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

Fig2: Western blot analysis of PTGER2 on different lysates with Rabbit anti-PTGER2 antibody (HA721380) at 1/1,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: A549 cell lysate
Lane 3: THP-1 cell lysate

Lysates/proteins at 15 µg/Lane.

Predicted band size: 40 kDa
Observed band size: 50 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721380) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

	Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-PTGER2 antibody (HA721380) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721380) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-PTGER2 antibody (HA721380) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA721380) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunocytochemistry analysis of JAR cells labeling PTGER2 with Rabbit anti-PTGER2 antibody (HA721380) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PTGER2 antibody (HA721380) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Fig6: Flow cytometric analysis of JAR cells labeling PTGER2.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721380, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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