Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE31-24 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 46 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within Human DC-SIGN aa 1-100 / 404. |
Positive control: | A549 cell lysate, human skin tissue lysate, human placenta tissue lysate. |
Subcellular location: | Cell membrane; Secreted. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: Q9NNX6 Human |
Alternative names: | C type lectin domain family 4 member L C-type lectin domain family 4 member L CD 209 CD209 CD209 antigen CD209 antigen-like protein A CD209 molecule CD209_HUMAN Cd209a CDSIGN CIRE CLEC4L DC SIGN DC SIGN1 DC-SIGN DC-SIGN1 DCSIGN Dendritic cell specific ICAM 3 grabbing nonintegrin 1 Dendritic cell specific ICAM3 grabbing nonintegrin 1 Dendritic cell-specific ICAM-3-grabbing non-integrin 1 Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin Dengue fever, protection against, included Dentritic Cell Specific ICAM3 Grabbing Nonintegrin HIV GP120 Binding Protein MGC129965 MGC130443 SIGN-R1 SIGNR5 |
Fig1:
Western blot analysis of DC-SIGN on different lysates with Rabbit anti-DC-SIGN antibody (HA721382) at 1/1,000 dilution. Lane 1: A549 cell lysate (15 µg/Lane) Lane 2: Human skin tissue lysate (30 µg/Lane) Lane 3: Human placenta tissue lysate (30 µg/Lane) Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721382) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |