DC-SIGN Recombinant Rabbit Monoclonal Antibody [JE31-24]
cat.: HA721382
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE31-24
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 46 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human DC-SIGN aa 1-100 / 404.
Positive control: A549 cell lysate, human skin tissue lysate, human placenta tissue lysate.
Subcellular location: Cell membrane; Secreted.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q9NNX6 Human
Alternative names: C type lectin domain family 4 member L C-type lectin domain family 4 member L CD 209 CD209 CD209 antigen CD209 antigen-like protein A CD209 molecule CD209_HUMAN Cd209a CDSIGN CIRE CLEC4L DC SIGN DC SIGN1 DC-SIGN DC-SIGN1 DCSIGN Dendritic cell specific ICAM 3 grabbing nonintegrin 1 Dendritic cell specific ICAM3 grabbing nonintegrin 1 Dendritic cell-specific ICAM-3-grabbing non-integrin 1 Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin Dengue fever, protection against, included Dentritic Cell Specific ICAM3 Grabbing Nonintegrin HIV GP120 Binding Protein MGC129965 MGC130443 SIGN-R1 SIGNR5
Images
HA721382_1.jpg Fig1: Western blot analysis of DC-SIGN on different lysates with Rabbit anti-DC-SIGN antibody (HA721382) at 1/1,000 dilution.

Lane 1: A549 cell lysate (15 µg/Lane)
Lane 2: Human skin tissue lysate (30 µg/Lane)
Lane 3: Human placenta tissue lysate (30 µg/Lane)

Predicted band size: 46 kDa
Observed band size: 46 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721382) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.