NKX3.1 Recombinant Rabbit Monoclonal Antibody [JE36-27]
cat.: HA721383
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE36-27 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 26 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human NKX3.1 aa 1-234 / 234. |
| Positive control: | A549 cell lysate, RWPE-1 cell lysate, LNCaP cell lysate, human prostate tissue, human prostate carcinoma tissue, human testis tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:1,000 1:200-1:1,000 |
| Uniprot #: | SwissProt: Q99801 Human |
| Alternative names: | BAPX 2 BAPX2 Homeobox protein NK-3 homolog A Homeobox protein Nkx 3.1 Homeobox protein Nkx-3.1 Homeobox protein Nkx3.1 NK homeobox (Drosophila) family 3 A NK homeobox family 3 A NK homeobox, family 3, member A NK3 homeobox 1 NK3 transcription factor homolog A NK3 transcription factor related locus 1 NKX 3 Nkx 3.1 NKX 3A NKX3 1 NKX3 Nkx3-1 NKX3.1 Nkx3.1, mouse, homolog of NKX31_HUMAN NKX3A |
Images
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Fig1:
Western blot analysis of NKX3.1 on different lysates with Rabbit anti-NKX3.1 antibody (HA721383) at 1/1,000 dilution. Lane 1: A549 cell lysate Lane 2: RWPE-1 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 26 kDa Observed band size: 30 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721383) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of NKX3.1 on different lysates with Rabbit anti-NKX3.1 antibody (HA721383) at 1/500 dilution. Lane 1: LNCaP cell lysate Lane 2: PC-3M cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 26 kDa Observed band size: 30 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721383) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-NKX3.1 antibody (HA721383) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721383) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-NKX3.1 antibody (HA721383) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721383) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig5:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-NKX3.1 antibody (HA721383) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721383) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.