UBE2T Recombinant Rabbit Monoclonal Antibody [JE65-79]
cat.: HA721385
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE65-79 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22.5 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human UBE2T aa 1-100 / 197. |
| Positive control: | HeLa cell lysate, K-562 cell lysate, MCF7 cell lysate, A549 cell lysate, HEK-293 cell lysate, Jurkat cell lysate, SKOV-3 cell lysate, SH-SY5Y cell lysate, HepG2 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, HeLa. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q9NPD8 Human | Q9CQ37 Mouse Entrez Gene: 360847 Rat |
| Alternative names: | Cell proliferation inducing gene 50 protein Cell proliferation-inducing gene 50 protein HSPC150 HSPC150 protein similar to ubiquitin conjugating enzyme PIG50 Ube2t UBE2T_HUMAN Ubiquitin carrier protein T Ubiquitin conjugating enzyme ubiquitin conjugating enzyme E2 T Ubiquitin conjugating enzyme E2T Ubiquitin protein ligase T Ubiquitin-conjugating enzyme E2 T Ubiquitin-protein ligase T |
Images
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Fig1:
Western blot analysis of UBE2T on different lysates with Rabbit anti-UBE2T antibody (HA721385) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: K-562 cell lysate (15 µg/Lane) Lane 3: MCF7 cell lysate (15 µg/Lane) Lane 4: A549 cell lysate (15 µg/Lane) Lane 5: HEK-293 cell lysate (15 µg/Lane) Lane 6: Jurkat cell lysate (15 µg/Lane) Lane 7: SKOV-3 cell lysate (15 µg/Lane) Lane 8: SH-SY5Y cell lysate (15 µg/Lane) Lane 9: HepG2 cell lysate (15 µg/Lane) Lane 10: Mouse testis tissue lysate (30 µg/Lane) Lane 11: Rat testis tissue lysate (30 µg/Lane) Predicted band size: 22.5 kDa Observed band size: 22.5 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721385) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunocytochemistry analysis of HeLa cells labeling UBE2T with Rabbit anti-UBE2T antibody (HA721385) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-UBE2T antibody (HA721385) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.