UBE2T Recombinant Rabbit Monoclonal Antibody [JE65-79]
cat.: HA721385
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell
Clonality: Monoclonal
Clone number: JE65-79
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 22.5 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human UBE2T aa 1-100 / 197.
Positive control: HeLa cell lysate, K-562 cell lysate, MCF7 cell lysate, A549 cell lysate, HEK-293 cell lysate, Jurkat cell lysate, SKOV-3 cell lysate, SH-SY5Y cell lysate, HepG2 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, HeLa.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell

1:1,000
1:100
Uniprot #: SwissProt: Q9NPD8 Human | Q9CQ37 Mouse
Entrez Gene: 360847 Rat
Alternative names: Cell proliferation inducing gene 50 protein Cell proliferation-inducing gene 50 protein HSPC150 HSPC150 protein similar to ubiquitin conjugating enzyme PIG50 Ube2t UBE2T_HUMAN Ubiquitin carrier protein T Ubiquitin conjugating enzyme ubiquitin conjugating enzyme E2 T Ubiquitin conjugating enzyme E2T Ubiquitin protein ligase T Ubiquitin-conjugating enzyme E2 T Ubiquitin-protein ligase T
Images
HA721385_1.jpg Fig1: Western blot analysis of UBE2T on different lysates with Rabbit anti-UBE2T antibody (HA721385) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: K-562 cell lysate (15 µg/Lane)
Lane 3: MCF7 cell lysate (15 µg/Lane)
Lane 4: A549 cell lysate (15 µg/Lane)
Lane 5: HEK-293 cell lysate (15 µg/Lane)
Lane 6: Jurkat cell lysate (15 µg/Lane)
Lane 7: SKOV-3 cell lysate (15 µg/Lane)
Lane 8: SH-SY5Y cell lysate (15 µg/Lane)
Lane 9: HepG2 cell lysate (15 µg/Lane)
Lane 10: Mouse testis tissue lysate (30 µg/Lane)
Lane 11: Rat testis tissue lysate (30 µg/Lane)

Predicted band size: 22.5 kDa
Observed band size: 22.5 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721385) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721385_2.jpg Fig2: Immunocytochemistry analysis of HeLa cells labeling UBE2T with Rabbit anti-UBE2T antibody (HA721385) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-UBE2T antibody (HA721385) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.