EXOC2 Recombinant Rabbit Monoclonal Antibody [JE65-98]
cat.: HA721386
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE65-98 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 104 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human EXOC2 aa 1-100 / 924. |
| Positive control: | K-562 cell lysate, Jurkat cell lysate, HepG2 cell lysate, MCF7 cell lysate, MDA-MB-231 cell lysate, HeLa cell lysate, HEK-293 cell lysate, U-2 OS cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate. |
| Subcellular location: | Midbody, Midbody ring. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q96KP1 Human | Q9D4H1 Mouse | O54921 Rat |
| Alternative names: | EXOC2 EXOC2_HUMAN Exocyst complex component 2 Exocyst complex component Sec5 FLJ11026 SEC5 SEC5 like 1 SEC5L1 Sec5p |
Images
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Fig1:
Western blot analysis of EXOC2 on different lysates with Rabbit anti-EXOC2 antibody (HA721386) at 1/1,000 dilution. Lane 1: K-562 cell lysate (15 µg/Lane) Lane 2: Jurkat cell lysate (15 µg/Lane) Lane 3: HepG2 cell lysate (15 µg/Lane) Lane 4: MCF7 cell lysate (15 µg/Lane) Lane 5: MDA-MB-231 cell lysate (15 µg/Lane) Lane 6: HeLa cell lysate (15 µg/Lane) Lane 7: HEK-293 cell lysate (15 µg/Lane) Lane 8: U-2 OS cell lysate (15 µg/Lane) Lane 9: Human brain tissue lysate (30 µg/Lane) Lane 10: Mouse brain tissue lysate (30 µg/Lane) Lane 11: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 104 kDa Observed band size: 104 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721386) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.