RAB22A Recombinant Rabbit Monoclonal Antibody [JE66-08]
cat.: HA721387
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell
Clonality: Monoclonal
Clone number: JE66-08
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 22 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human RAB22A aa 1-100 / 194.
Positive control: HepG2 cell lysate, K-562 cell lysate, HeLa cell lysate, MCF7 cell lysate, Jurkat cell lysate, U-2 OS cell lysate, A549 cell lysate, HEK-293 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse kidney tissue lysate, mouse testis tissue lysate, Rat liver testis lysate, MCF7.
Subcellular location: Endosome membrane, Cell membrane, Early endosome, Late endosome, Cell projection, ruffle, Cytoplasmic vesicle, Cytoplasmic vesicle, phagosome, phagosome membrane.
Recommended Dilutions:
  WB
  IF-Cell

1:1,000
1:100
Uniprot #: SwissProt: Q9UL26 Human | P35285 Mouse
Entrez Gene: 366265 Rat
Alternative names: 3732413A17Rik AI662177 AW319644 AW550514 E130120E14Rik GTP binding protein RAB22A MGC16770 OTTMUSP00000017605 Rab 22 RAB 22A Rab-22 Rab22 RAB22A RAB22A member RAS oncogene family Ras related protein Rab 22A Ras related protein Rab22A Ras-related protein Rab-22A RB22A_HUMAN
Images
HA721387_1.jpg Fig1: Western blot analysis of RAB22A on different lysates with Rabbit anti-RAB22A antibody (HA721387) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate (15 µg/Lane)
Lane 2: K-562 cell lysate (15 µg/Lane)
Lane 3: HeLa cell lysate (15 µg/Lane)
Lane 4: MCF7 cell lysate (15 µg/Lane)
Lane 5: Jurkat cell lysate (15 µg/Lane)
Lane 6: U-2 OS cell lysate (15 µg/Lane)
Lane 7: A549 cell lysate (15 µg/Lane)
Lane 8: HEK-293 cell lysate (15 µg/Lane)
Lane 9: Mouse brain tissue lysate (30 µg/Lane)
Lane 10: Rat brain tissue lysate (30 µg/Lane)
Lane 11: Mouse kidney tissue lysate (30 µg/Lane)
Lane 12: Mouse testis tissue lysate (30 µg/Lane)
Lane 13: Rat liver testis lysate (30 µg/Lane)

Predicted band size: 22 kDa
Observed band size: 20 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721387) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721387_2.jpg Fig2: Immunocytochemistry analysis of MCF7 cells labeling RAB22A with Rabbit anti-RAB22A antibody (HA721387) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RAB22A antibody (HA721387) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.