RAB22A Recombinant Rabbit Monoclonal Antibody [JE66-08]
cat.: HA721387
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | JE66-08 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 22 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human RAB22A aa 1-100 / 194. |
| Positive control: | HepG2 cell lysate, K-562 cell lysate, HeLa cell lysate, MCF7 cell lysate, Jurkat cell lysate, U-2 OS cell lysate, A549 cell lysate, HEK-293 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, mouse kidney tissue lysate, mouse testis tissue lysate, Rat liver testis lysate, MCF7. |
| Subcellular location: | Endosome membrane, Cell membrane, Early endosome, Late endosome, Cell projection, ruffle, Cytoplasmic vesicle, Cytoplasmic vesicle, phagosome, phagosome membrane. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:100 |
| Uniprot #: | SwissProt: Q9UL26 Human | P35285 Mouse Entrez Gene: 366265 Rat |
| Alternative names: | 3732413A17Rik AI662177 AW319644 AW550514 E130120E14Rik GTP binding protein RAB22A MGC16770 OTTMUSP00000017605 Rab 22 RAB 22A Rab-22 Rab22 RAB22A RAB22A member RAS oncogene family Ras related protein Rab 22A Ras related protein Rab22A Ras-related protein Rab-22A RB22A_HUMAN |
Images
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Fig1:
Western blot analysis of RAB22A on different lysates with Rabbit anti-RAB22A antibody (HA721387) at 1/1,000 dilution. Lane 1: HAP1-parental cell lysate (10 µg/Lane) Lane 2: HAP1-RAB22A KD cell lysate (10 µg/Lane) Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 120 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721387) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of RAB22A on different lysates with Rabbit anti-RAB22A antibody (HA721387) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (15 µg/Lane) Lane 2: K-562 cell lysate (15 µg/Lane) Lane 3: HeLa cell lysate (15 µg/Lane) Lane 4: MCF7 cell lysate (15 µg/Lane) Lane 5: Jurkat cell lysate (15 µg/Lane) Lane 6: U-2 OS cell lysate (15 µg/Lane) Lane 7: A549 cell lysate (15 µg/Lane) Lane 8: HEK-293 cell lysate (15 µg/Lane) Lane 9: Mouse brain tissue lysate (30 µg/Lane) Lane 10: Rat brain tissue lysate (30 µg/Lane) Lane 11: Mouse kidney tissue lysate (30 µg/Lane) Lane 12: Mouse testis tissue lysate (30 µg/Lane) Lane 13: Rat liver testis lysate (30 µg/Lane) Predicted band size: 22 kDa Observed band size: 20 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721387) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of MCF7 cells labeling RAB22A with Rabbit anti-RAB22A antibody (HA721387) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-RAB22A antibody (HA721387) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.