ESE1 Recombinant Rabbit Monoclonal Antibody [JE33-30]
cat.: HA721388
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE33-30 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41.5 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ESE1 aa 1-371 / 371. |
| Positive control: | A549 cell lysate, SK-Br-3 cell lysate, A431 cell lysate, mouse lung tissue lysate, rat kidney tissue lysate, A431. |
| Subcellular location: | Cytoplasm, Nucleus. |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P78545 Human | Q3UPW2 Mouse | Q4V7E1 Rat |
| Alternative names: | E74 like factor 3 E74 like factor 3 ets domain transcription factor E74 like factor 3 ets domain transcription factor epithelial specific E74 like factor 3 ETS domain transcription factor serine box epithelial specific E74-like factor 3 Elf3 ELF3_HUMAN Epithelial restricted with serine box Epithelial-restricted with serine box Epithelium restricted Ets protein ESX Epithelium specific Ets factor 1 Epithelium specific Ets transcription factor 1 Epithelium-restricted Ets protein ESX Epithelium-specific Ets transcription factor 1 EPR 1 EPR1 ERT ESE-1 ESX Ets domain transcription factor serine box Ets domain transcription factor serine box epithelial specific Ets transcription factor ETS-related transcription factor Elf-3 jen MGC139318 |
Images
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Fig1:
Western blot analysis of ESE1 on different lysates with Rabbit anti-ESE1 antibody (HA721388) at 1/1,000 dilution. Lane 1: A549 cell lysate (10 µg/Lane) Lane 2: SK-Br-3 cell lysate (10 µg/Lane) Lane 3: A431 cell lysate (10 µg/Lane) Lane 4: Mouse lung tissue lysate (20 µg/Lane) Lane 5: Rat kidney tissue lysate (20 µg/Lane) Predicted band size: 41.5 kDa Observed band size: 41.5 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721388) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of A431 cells labeling ESE1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721388, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.