ESE1 Recombinant Rabbit Monoclonal Antibody [JE33-30]
cat.: HA721388
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, FC
Clonality: Monoclonal
Clone number: JE33-30
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 41.5 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human ESE1 aa 1-371 / 371.
Positive control: A549 cell lysate, SK-Br-3 cell lysate, A431 cell lysate, mouse lung tissue lysate, rat kidney tissue lysate, A431.
Subcellular location: Cytoplasm, Nucleus.
Recommended Dilutions:
  WB
  FC

1:1,000
1:500-1:1,000
Uniprot #: SwissProt: P78545 Human | Q3UPW2 Mouse | Q4V7E1 Rat
Alternative names: E74 like factor 3 E74 like factor 3 ets domain transcription factor E74 like factor 3 ets domain transcription factor epithelial specific E74 like factor 3 ETS domain transcription factor serine box epithelial specific E74-like factor 3 Elf3 ELF3_HUMAN Epithelial restricted with serine box Epithelial-restricted with serine box Epithelium restricted Ets protein ESX Epithelium specific Ets factor 1 Epithelium specific Ets transcription factor 1 Epithelium-restricted Ets protein ESX Epithelium-specific Ets transcription factor 1 EPR 1 EPR1 ERT ESE-1 ESX Ets domain transcription factor serine box Ets domain transcription factor serine box epithelial specific Ets transcription factor ETS-related transcription factor Elf-3 jen MGC139318
Images
HA721388_1.jpg Fig1: Western blot analysis of ESE1 on different lysates with Rabbit anti-ESE1 antibody (HA721388) at 1/1,000 dilution.

Lane 1: A549 cell lysate (10 µg/Lane)
Lane 2: SK-Br-3 cell lysate (10 µg/Lane)
Lane 3: A431 cell lysate (10 µg/Lane)
Lane 4: Mouse lung tissue lysate (20 µg/Lane)
Lane 5: Rat kidney tissue lysate (20 µg/Lane)

Predicted band size: 41.5 kDa
Observed band size: 41.5 kDa

Exposure time: 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721388) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721388_2.jpg Fig2: Flow cytometric analysis of A431 cells labeling ESE1.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721388, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.