HRPT2 Recombinant Rabbit Monoclonal Antibody [JE66-42]
cat.: HA721390
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE66-42 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 61 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HRPT2 aa 331-430 / 531. |
| Positive control: | SW480 cell lysate, K-562 cell lysate, HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, MCF7 cell lysate, HEK-293 cell lysate, RAW264.7 cell lysate, human kidney tissue, human testis tissue, rat kidney tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: Q6P1J9 Human | Q8JZM7 Mouse | Q4V8C8 Rat |
| Alternative names: | C1orf28 CDC 73 Cdc73 CDC73_HUMAN Cell division cycle 73 Cell division cycle 73 Paf1/RNA polymerase II complex component homolog Cell division cycle 73 Paf1/RNA polymerase II complex component like protein Cell division cycle protein 73 homolog FIHP FLJ23316 HPT JT HPTJT HRPT 2 HRPT1 HRPT2 Hyperparathyroidism 2 (with jaw tumor) Hyperparathyroidism 2 Hyperparathyroidism 2 protein HYX Paf1/RNA polymerase II complex component Parafibromin |
Images
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Fig1:
Western blot analysis of HRPT2 on different lysates with Rabbit anti-HRPT2 antibody (HA721390) at 1/1,000 dilution. Lane 1: SW480 cell lysate Lane 2: K-562 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: MCF7 cell lysate Lane 7: HEK-293 cell lysate Lane 8: RAW264.7 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 61 kDa Observed band size: 61 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721390) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HRPT2 antibody (HA721390) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721390) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-HRPT2 antibody (HA721390) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721390) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig4:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-HRPT2 antibody (HA721390) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721390) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.