Dopamine Receptor D3 Recombinant Rabbit Monoclonal Antibody [JE33-33]
cat.: HA721391
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE33-33 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Dopamine Receptor D3 aa 1-50 / 400. |
| Positive control: | HeLa cell lysate, SH-SY5Y cell lysate, mouse brain tissue lysate, rat brain tissue lysate. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P35462 Human | P30728 Mouse | P19020 Rat |
| Alternative names: | 3dopamine receptor D D(3) dopamine receptor Dopamine D3 receptor Dopamine receptor D3 DRD3 DRD3_HUMAN ETM1 FET1 |
Images
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Fig1:
Western blot analysis of Dopamine Receptor D3 on different lysates with Rabbit anti-Dopamine Receptor D3 antibody (HA721391) at 1/1,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: SH-SY5Y cell lysate (10 µg/Lane) Lane 3: Mouse brain tissue lysate (20 µg/Lane) Lane 4: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 44 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721391) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.