TNKS1 Recombinant Rabbit Monoclonal Antibody [PSH0-51]
cat.: HA721392
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH0-51 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 142 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human TNKS1 aa 151-650/1,327. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, MCF7 cell lysate, Raji cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate. |
| Subcellular location: | Cytoplasm, Golgi apparatus membrane, cytoskeleton, microtubule organizing center, centrosome, Nucleus, nuclear pore complex, Chromosome, telomere, cytoskeleton, spindle pole. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O95271 Human |
| Alternative names: | ADP-ribosyltransferase diphtheria toxin-like 5 OTTHUMP00000115550 OTTHUMP00000224675 PARP 5a PARP5A PARPL pART5 Poly [ADP-ribose] polymerase 5A TANK 1 TANK1 Tankyrase 1 Tankyrase I Tankyrase TRF1 interacting ankyrin related ADP ribose polymerase Tankyrase-1 Tankyrase1 TankyraseI TIN 1 TIN1 TINF 1 TINF1 TNKS 1 TNKS TNKS-1 TNKS1 TNKS1_HUMAN TRF1 interacting ankyrin related ADP ribose polymerase TRF1-interacting ankyrin-related ADP-ribose polymerase |
Images
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Fig1:
Western blot analysis of TNKS1 on different lysates with Rabbit anti-TNKS1 antibody (HA721392) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF7 cell lysate Lane 4: Raji cell lysate Lane 5: HepG2 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 142 kDa Observed band size: 150 kDa Exposure time: 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721392) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of TNKS1 on different lysates with Rabbit anti-TNKS1 antibody (HA721392) at 1/1,000 dilution. Lane 1: HeLa-si NT cell lysate Lane 2: HeLa-si TNKS1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 142 kDa Observed band size: 150 kDa Exposure time: 4 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721392) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.