Adenosine A1 Receptor Recombinant Rabbit Monoclonal Antibody [JE31-35]
cat.: HA721393
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE31-35 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 36.5 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Adenosine A1 Receptor aa 103-201 / 326. |
| Positive control: | A549 cell lysate, HEK-293 cell lysate, U-87 MG cell lysate, SH-SY5Y cell lysate, HeLa cell lysate, Raji cell lysate, NIH/3T3 cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Human cerebellum tissue lysate, Rat cerebellum tissue lysate, human brain tissue, human cerebellum tissue, mouse brain tissue, mouse cerebellum tissue, mouse hippocampus tissue, rat brain tissue, rat cerebellum tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: P30542 Human | Q60612 Mouse | P25099 Rat |
| Alternative names: | A1AR A1R AA1R AA1R_HUMAN Adenosine receptor A1 ADORA 1 ADORA 1 ADORA1 RDC 7 RDC7 Ri |
Images
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Fig1:
Western blot analysis of Adenosine A1 Receptor on different lysates with Rabbit anti-Adenosine A1 Receptor antibody (HA721393) at 1/1,000 dilution. Lane 1: A549 cell lysate (15 µg/Lane) Lane 2: HEK-293 cell lysate (15 µg/Lane) Lane 3: U-87 MG cell lysate (15 µg/Lane) Lane 4: SH-SY5Y cell lysate (15 µg/Lane) Lane 5: HeLa cell lysate (15 µg/Lane) Lane 6: Raji cell lysate (15 µg/Lane) Lane 7: NIH/3T3 cell lysate (15 µg/Lane) Lane 8: Human brain tissue lysate (30 µg/Lane) Lane 9: Mouse brain tissue lysate (30 µg/Lane) Lane 10: Rat brain tissue lysate (30 µg/Lane) Lane 11: Human cerebellum tissue lysate (30 µg/Lane) Lane 12: Rat cerebellum tissue lysate (30 µg/Lane) Predicted band size: 36.5 kDa Observed band size: 36.5/40 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721393) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Adenosine A1 Receptor antibody (HA721393) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721393) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rabbit anti-Adenosine A1 Receptor antibody (HA721393) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721393) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Adenosine A1 Receptor antibody (HA721393) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721393) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rabbit anti-Adenosine A1 Receptor antibody (HA721393) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721393) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Adenosine A1 Receptor antibody (HA721393) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721393) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Adenosine A1 Receptor antibody (HA721393) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721393) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Adenosine A1 Receptor antibody (HA721393) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721393) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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