Dihydrofolate reductase Recombinant Rabbit Monoclonal Antibody [JE31-05]
cat.: HA721394
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE31-05 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 21.5 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Dihydrofolate reductase aa 88-187 / 187. |
| Positive control: | HCT116 cell lysate, HeLa cell lysate, U-2 OS cell lysate, K-562 cell lysate, MCF7 cell lysate, HEK-293 cell lysate, Jurkat cell lysate, HepG2 cell lysate, LO2 cell lysate, mouse liver tissue lysate, rat liver tissue lysate, human colon carcinoma tissue, human liver tissue. |
| Subcellular location: | Mitochondrion, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P00374 Human | P00375 Mouse | Q920D2 Rat |
| Alternative names: | DHFR DHFRP1 Dihydrofolate reductase DYR DYR_HUMAN EC 1.5.1.3 |
Images
|
Fig1:
Western blot analysis of Dihydrofolate reductase on different lysates with Rabbit anti-Dihydrofolate reductase antibody (HA721394) at 1/1,000 dilution. Lane 1: HCT116 cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: U-2 OS cell lysate (15 µg/Lane) Lane 4: K-562 cell lysate (15 µg/Lane) Lane 5: MCF7 cell lysate (15 µg/Lane) Lane 6: HEK-293 cell lysate (15 µg/Lane) Lane 7: Jurkat cell lysate (15 µg/Lane) Lane 8: HepG2 cell lysate (15 µg/Lane) Lane 9: LO2 cell lysate (15 µg/Lane) Lane 10: Mouse liver tissue lysate (30 µg/Lane) Lane 11: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 21.5 kDa Observed band size: 21.5 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721394) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Dihydrofolate reductase antibody (HA721394) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721394) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Dihydrofolate reductase antibody (HA721394) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721394) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.