HEC1 Recombinant Rabbit Monoclonal Antibody [JE31-10]
cat.: HA721395
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE31-10 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 74 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HEC1 aa 593-642 / 642. |
| Positive control: | K-562 cell lysate, U-2 OS cell lysate, HeLa cell lysate, HEK-293 cell lysate, Jurkat cell lysate, MCF7 cell lysate, HepG2 cell lysate, LO2 cell lysate. |
| Subcellular location: | Nucleus, Chromosome, centromere, kinetochore. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: O14777 Human |
| Alternative names: | from Entrez Gene ; HEC Highly expressed in cancer Highly expressed in cancer protein Highly expressed in cancer rich in leucine heptad repeats HsHec1 hsNDC80 Kinetochore associated 2 Kinetochore associated protein 2 Kinetochore protein Hec1 Kinetochore protein NDC80 homolog Kinetochore-associated protein 2 KNTC2 ndc80 NDC80 homolog kinetochore complex component NDC80 kinetochore complex component homolog NDC80, S. cerevisiae, homolog of NDC80_HUMAN Retinoblastoma associated protein HEC Retinoblastoma-associated protein HEC TID3 |
Images
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Fig1:
Western blot analysis of HEC1 on different lysates with Rabbit anti-HEC1 antibody (HA721395) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: U-2 OS cell lysate Lane 3: HeLa cell lysate Lane 4: HEK-293 cell lysate Lane 5: Jurkat cell lysate Lane 6: MCF7 cell lysate Lane 7: HepG2 cell lysate Lane 8: LO2 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 74 kDa Observed band size: 74 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721395) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.