Alpha 1 Acid Glycoprotein Recombinant Rabbit Monoclonal Antibody [JE31-16]
cat.: HA721396
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE31-16 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 23.5 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Alpha 1 Acid Glycoprotein aa 51-100 / 201. |
| Positive control: | Human small intestine tissue lysates, human liver tissue. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:200 |
| Uniprot #: | SwissProt: P02763 Human |
| Alternative names: | A1AG1_HUMAN AGP 1 AGP A AGP AGP1 Alpha 1 acid glycoprotein Alpha-1-acid glycoprotein 1 alpha-1-AGP Epididymis secretory sperm binding protein Li 153w glycoprotein, alpha-1-acid, of serum HEL S 153w OMD 1 ORM ORM1 Orosomucoid 1 Orosomucoid-1 |
Images
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Fig1:
Western blot analysis of Alpha 1 Acid Glycoprotein on human small intestine tissue lysates with Rabbit anti-Alpha 1 Acid Glycoprotein antibody (HA721396) at 1/1,000 dilution. Lysates/proteins at 30 µg/Lane. Predicted band size: 23.5 kDa Observed band size: 45 kDa (Glycosylation) Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721396) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Alpha 1 Acid Glycoprotein antibody (HA721396) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721396) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.