Chromogranin B Recombinant Rabbit Monoclonal Antibody [JE31-72]
cat.: HA721398
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE31-72 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 78 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Chromogranin B aa 601-650 / 677. |
| Positive control: | 293T cell lysate, SH-SY5Y cell lysate, SW620 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, HeLa cell lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P05060 Human | P16014 Mouse | O35314 Rat |
| Alternative names: | CCB peptide Cg B CgB Chgb Chromogranin-B ChromograninB SCG1 SCG1_HUMAN Secretogranin 1 Secretogranin B Secretogranin I SgI |
Images
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Fig1:
Western blot analysis of Chromogranin B on different lysates with Rabbit anti-Chromogranin B antibody (HA721398) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: SW620 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 78 kDa Observed band size: 120 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721398) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Chromogranin B on different lysates with Rabbit anti-Chromogranin B antibody (HA721398) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: RAW264.7 cell lysate Lane 3: PC-12 cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 78 kDa Observed band size: 120 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721398) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.