PSMD13 Recombinant Rabbit Monoclonal Antibody [PSH0-52]
cat.: HA721399
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH0-52 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 43 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PSMD13 aa 1-150/376. |
| Positive control: | HeLa cell lysate, Jurkat cell lysate, HepG2 cell lysate, SiHa cell lysate, HEK-293 cell lysate, SH-SY5Y cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, human brain tissue, rat brain tissue, HeLa. |
| Subcellular location: | Proteasome, cytosol, extracellular region, nucleoplasm, nucleus. |
| Recommended Dilutions:
WB IHC-P IF-Cell |
1:1,000 1:200 1:200 |
| Uniprot #: | SwissProt: Q9UNM6 Human | Q9WVJ2 Mouse | B0BN93 Rat |
| Alternative names: | 26S proteasome non ATPase regulatory subunit 13 26S proteasome non-ATPase regulatory subunit 13 26S proteasome regulatory subunit p40.5 26S proteasome regulatory subunit RPN9 26S proteasome regulatory subunit S11 26S proteasome subunit p40.5 HSPC027 P40.5 Proteasome (prosome, macropain) 26S subunit, non ATPase, 13 Proteasome 26S subunit, non-ATPase 13 PSD13_HUMAN psmD13 Rpn9 S11 |
Images
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Fig1:
Western blot analysis of PSMD13 on different lysates with Rabbit anti-PSMD13 antibody (HA721399) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: HepG2 cell lysate Lane 4: SiHa cell lysate Lane 5: HEK-293 cell lysate Lane 6: SH-SY5Y cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Mouse testis tissue lysate Lane 10: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721399) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PSMD13 antibody (HA721399) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721399) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-PSMD13 antibody (HA721399) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721399) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of HeLa cells labeling PSMD13 with Rabbit anti-PSMD13 antibody (HA721399) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-PSMD13 antibody (HA721399) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.