Tankyrase 2 Recombinant Rabbit Monoclonal Antibody [PSH0-53]
cat.: HA721400
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IF-Cell
Clonality: Monoclonal
Clone number: PSH0-53
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 127 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Tankyrase 2 aa 451-700/1,166.
Positive control: PC-12 treated with 1μM XAV939 for 24 hours whole cell lysate, PC-12 treated with 1μM XAV939 for 48 hours whole cell lysate, A431.
Subcellular location: Cytoplasm, Golgi apparatus membrane, Nucleus, Chromosome, telomere.
Recommended Dilutions:
  WB
  IF-Cell
1:1,000
1:250
Uniprot #: SwissProt: Q9H2K2 Human
Entrez Gene: 309512 Rat
Alternative names: ADP-ribosyltransferase diphtheria toxin-like 6 ARTD6 PARP 5b PARP 5c PARP-5b PARP-5c PARP5B PARP5C pART6 Poly [ADP ribose] polymerase 5B Poly [ADP-ribose] polymerase 5B TANK2 Tankyrase 2 Tankyrase II Tankyrase like protein Tankyrase related protein Tankyrase TRF1 interacting ankyrin related ADP ribose polymerase 2 Tankyrase-2 Tankyrase-like protein Tankyrase-related protein TNKL TNKS 2 TNKS-2 TNKS2 TNKS2_HUMAN TRF1 Interacting Ankyrin Related ADP ribose polymerase 2 TRF1-interacting ankyrin-related ADP-ribose polymerase 2
Images
HA721400_1.jpg Fig1: Western blot analysis of Tankyrase 2 on different lysates with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/1,000 dilution.

Lane 1: PC-12 whole cell lysate
Lane 2: PC-12 treated with 1μM XAV939 for 24 hours whole cell lysate
Lane 3: PC-12 treated with 1μM XAV939 for 48 hours whole cell lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 127 kDa
Observed band size: 127 kDa

Exposure time: 10 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721400) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721400_2.jpg Fig2: Western blot analysis of Tankyrase 2 on different lysates with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/2,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Tankyrase 2 KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 127 kDa
Observed band size: 90 kDa

Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721400) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
HA721400_3.jpg Fig3: Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.