Tankyrase 2 Recombinant Rabbit Monoclonal Antibody [PSH0-53]
cat.: HA721400
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IF-Cell |
| Clonality: | Monoclonal |
| Clone number: | PSH0-53 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 127 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Tankyrase 2 aa 451-700/1,166. |
| Positive control: | PC-12 treated with 1μM XAV939 for 24 hours whole cell lysate, PC-12 treated with 1μM XAV939 for 48 hours whole cell lysate, A431. |
| Subcellular location: | Cytoplasm, Golgi apparatus membrane, Nucleus, Chromosome, telomere. |
| Recommended Dilutions:
WB IF-Cell |
1:1,000 1:250 |
| Uniprot #: | SwissProt: Q9H2K2 Human Entrez Gene: 309512 Rat |
| Alternative names: | ADP-ribosyltransferase diphtheria toxin-like 6 ARTD6 PARP 5b PARP 5c PARP-5b PARP-5c PARP5B PARP5C pART6 Poly [ADP ribose] polymerase 5B Poly [ADP-ribose] polymerase 5B TANK2 Tankyrase 2 Tankyrase II Tankyrase like protein Tankyrase related protein Tankyrase TRF1 interacting ankyrin related ADP ribose polymerase 2 Tankyrase-2 Tankyrase-like protein Tankyrase-related protein TNKL TNKS 2 TNKS-2 TNKS2 TNKS2_HUMAN TRF1 Interacting Ankyrin Related ADP ribose polymerase 2 TRF1-interacting ankyrin-related ADP-ribose polymerase 2 |
Images
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Fig1:
Western blot analysis of Tankyrase 2 on different lysates with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/1,000 dilution. Lane 1: PC-12 whole cell lysate Lane 2: PC-12 treated with 1μM XAV939 for 24 hours whole cell lysate Lane 3: PC-12 treated with 1μM XAV939 for 48 hours whole cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 127 kDa Observed band size: 127 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721400) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Western blot analysis of Tankyrase 2 on different lysates with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/2,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-Tankyrase 2 KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 127 kDa Observed band size: 90 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721400) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunocytochemistry analysis of A431 cells labeling Tankyrase 2 with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Tankyrase 2 antibody (HA721400) at 1/250 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.