Notch2 Recombinant Rabbit Monoclonal Antibody [PSH0-54]
cat.: HA721401
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | PSH0-54 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 265 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Notch2 aa 2,151-2,350/2,471. |
| Positive control: | 293T cell lysate, HepG2 cell lysate, PC-3M cell lysate, U-87 MG cell lysate, HCT116 cell lysate, SW620 cell lysate. |
| Subcellular location: | Cell membrane, Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q04721 Human |
| Alternative names: | AGS2 hN2 Motch B N2 N2ECD N2ICD neurogenic locus notch homolog protein 2 NOTC2_HUMAN Notch 2 Notch 2 intracellular domain Notch Drosophila homolog 2 Notch homolog 2 Notch homolog 2 Drosophila Notch2 |
Images
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Fig1:
Western blot analysis of Notch2 on different lysates with Rabbit anti-Notch2 antibody (HA721401) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lane 3: PC-3M cell lysate Lane 4: U-87 MG cell lysate Lane 5: HCT116 cell lysate Lane 6: SW620 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 265 kDa Observed band size: 110 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721401) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.