Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse, Rat |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | PSH0-55 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 265 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human Notch2 aa 2,151-2,350/2,471. |
Positive control: | 293T cell lysate, HepG2 cell lysate, PC-3M cell lysate, U-87 MG cell lysate, HCT116 cell lysate. |
Subcellular location: | Cell membrane, Nucleus, Cytoplasm. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: Q04721 Human |
Alternative names: | AGS2 hN2 Motch B N2 N2ECD N2ICD neurogenic locus notch homolog protein 2 NOTC2_HUMAN Notch 2 Notch 2 intracellular domain Notch Drosophila homolog 2 Notch homolog 2 Notch homolog 2 Drosophila Notch2 |
Fig1:
Western blot analysis of Notch2 on different lysates with Rabbit anti-Notch2 antibody (HA721402) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HepG2 cell lysate Lane 3: PC-3M cell lysate Lane 4: U-87 MG cell lysate Lane 5: HCT116 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 265 kDa Observed band size: 110 kDa Exposure time: 2 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721402) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |