Notch2 Recombinant Rabbit Monoclonal Antibody [PSH0-55]
cat.: HA721402
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB
Clonality: Monoclonal
Clone number: PSH0-55
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 265 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Notch2 aa 2,151-2,350/2,471.
Positive control: 293T cell lysate, HepG2 cell lysate, PC-3M cell lysate, U-87 MG cell lysate, HCT116 cell lysate.
Subcellular location: Cell membrane, Nucleus, Cytoplasm.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: Q04721 Human
Alternative names: AGS2 hN2 Motch B N2 N2ECD N2ICD neurogenic locus notch homolog protein 2 NOTC2_HUMAN Notch 2 Notch 2 intracellular domain Notch Drosophila homolog 2 Notch homolog 2 Notch homolog 2 Drosophila Notch2
Images
HA721402_1.jpg Fig1: Western blot analysis of Notch2 on different lysates with Rabbit anti-Notch2 antibody (HA721402) at 1/1,000 dilution.

Lane 1: 293T cell lysate
Lane 2: HepG2 cell lysate
Lane 3: PC-3M cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: HCT116 cell lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 265 kDa
Observed band size: 110 kDa

Exposure time: 2 minutes 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721402) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.