LXR beta Recombinant Rabbit Monoclonal Antibody [PSH0-56]
cat.: HA721403
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell
Clonality: Monoclonal
Clone number: PSH0-56
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 51 kDa
Isotype: IgG
Immunogen: Synthetic peptide within human LXR beta aa 50-100/460.
Positive control: SW480 cell lysate, HepG2 cell lysate, PC-3M cell lysate, A549 cell lysate, HeLa cell lysate, HT-29 cell lysate, HCT116 cell lysate, HEK-293 cell lysate, mouse brain tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, C2C12, HCT116.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
1:1,000
1:100
Uniprot #: SwissProt: P55055 Human | Q60644 Mouse | Q62755 Rat
Alternative names: Liver X nuclear receptor beta Liver X receptor beta LX receptor beta LXR b LXR beta LXRB NER NER I NER1 NR1H 2 NR1H2 NR1H2_HUMAN Nuclear orphan receptor LXR beta Nuclear receptor NER Nuclear receptor subfamily 1 group H member 2 OR-1 Oxysterols receptor LXR-beta RIP15 Steroid hormone nuclear receptor NER Ubiquitously-expressed nuclear receptor UNR
Images
HA721403_1.jpg Fig1: Western blot analysis of LXR beta on different lysates with Rabbit anti-LXR beta antibody (HA721403) at 1/1,000 dilution.

Lane 1: SW480 cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: PC-3M cell lysate (20 µg/Lane)
Lane 4: A549 cell lysate (20 µg/Lane)
Lane 5: HeLa cell lysate (20 µg/Lane)
Lane 6: HT-29 cell lysate (20 µg/Lane)
Lane 7: HCT116 cell lysate (20 µg/Lane)
Lane 8: HEK-293 cell lysate (20 µg/Lane)
Lane 9: Mouse brain tissue lysate (40 µg/Lane)
Lane 10: Mouse liver tissue lysate (40 µg/Lane)
Lane 11: Rat liver tissue lysate (40 µg/Lane)

Predicted band size: 51 kDa
Observed band size: 51 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721403) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721403_2.jpg Fig2: Immunocytochemistry analysis of C2C12 cells labeling LXR beta with Rabbit anti-LXR beta antibody (HA721403) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LXR beta antibody (HA721403) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721403_3.jpg Fig3: Immunocytochemistry analysis of HCT116 cells labeling LXR beta with Rabbit anti-LXR beta antibody (HA721403) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LXR beta antibody (HA721403) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721403_4.jpg Fig4: Western blot analysis of LXR beta on different lysates with Rabbit anti-LXR beta antibody (HA721403) at 1/1,000 dilution.

Lane 1: HEK-293-si NT cell lysate
Lane 2: HEK-293-si LXR beta cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 51 kDa
Observed band size: 51 kDa

Exposure time: 2 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721403) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.