Ionotropic glutamate receptor 2 + 3 Recombinant Rabbit Monoclonal Antibody [JE33-28]
cat.: HA721405
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Mouse, Rat, Human
Applications: WB
Clonality: Monoclonal
Clone number: JE33-28
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 99 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human GRIA2 aa 834-883 / 883.
Positive control: Mouse brain tissue lysate, rat brain tissue lysate.
Subcellular location: Cell membrane, Endoplasmic reticulum membrane, Postsynaptic cell membrane, Postsynaptic density membrane.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P42262 Human | P42263 Human | P23819 Mouse | Q9Z2W9 Mouse | P19491 Rat | P19492 Rat
Alternative names: AMPA selective glutamate receptor 2 AMPA selective glutamate receptor 3 GluR K2 GluR K3 GLURB GLURC GLUTAMATE RECEPTOR 2 GLUTAMATE RECEPTOR 3 Glutamate receptor ionotropic AMPA 2 Glutamate receptor ionotropic AMPA 3 GRIA2 GRIA3
Images
HA721405_1.jpg Fig1: Western blot analysis of Ionotropic glutamate receptor 2 + 3 on different lysates with Rabbit anti-Ionotropic glutamate receptor 2 + 3 antibody (HA721405) at 1/1,000 dilution.

Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 99 kDa
Observed band size: 99 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721405) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.