| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat, Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE33-28 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 99 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human GRIA2 aa 834-883 / 883. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate. |
| Subcellular location: | Cell membrane, Endoplasmic reticulum membrane, Postsynaptic cell membrane, Postsynaptic density membrane. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P42262 Human | P42263 Human | P23819 Mouse | Q9Z2W9 Mouse | P19491 Rat | P19492 Rat |
| Alternative names: | AMPA selective glutamate receptor 2 AMPA selective glutamate receptor 3 GluR K2 GluR K3 GLURB GLURC GLUTAMATE RECEPTOR 2 GLUTAMATE RECEPTOR 3 Glutamate receptor ionotropic AMPA 2 Glutamate receptor ionotropic AMPA 3 GRIA2 GRIA3 |
|
Fig1:
Western blot analysis of Ionotropic glutamate receptor 2 + 3 on different lysates with Rabbit anti-Ionotropic glutamate receptor 2 + 3 antibody (HA721405) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 99 kDa Observed band size: 99 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721405) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |