Ran Recombinant Rabbit Monoclonal Antibody [JE33-28]
cat.: HA721406
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE33-28 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 24 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Ran aa 167-216 / 216. |
| Positive control: | A549 cell lysate, HeLa cell lysate, HepG2 cell lysate, LoVo cell lysate, Jurkat cell lysate, MDA-MB-231 cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, rat thymus tissue lysate, HeLa. |
| Subcellular location: | Nucleus, Nucleus envelope, Cytoplasm, cytosol, Melanosome. |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P62826 Human | P62827 Mouse | P62828 Rat |
| Alternative names: | Androgen receptor associated protein 24 Androgen receptor-associated protein 24 ARA 24 ARA24 Gsp1 GTP binding nuclear protein RAN GTP-binding nuclear protein Ran GTPase Ran Guanosine triphosphatase Ran LPS OK/SW-cl.81 ran RAN member RAS oncogene family RAN_HUMAN RanGTPase Ras like protein TC4 Ras related nuclear protein Ras-like protein TC4 Ras-related nuclear protein RASL2 8 TC 4 TC4 |
Images
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Fig1:
Western blot analysis of Ran on different lysates with Rabbit anti-Ran antibody (HA721406) at 1/1,000 dilution. Lane 1: A549 cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: HepG2 cell lysate (15 µg/Lane) Lane 4: LoVo cell lysate (15 µg/Lane) Lane 5: Jurkat cell lysate (15 µg/Lane) Lane 6: MDA-MB-231 cell lysate (15 µg/Lane) Lane 7: MCF7 cell lysate (15 µg/Lane) Lane 8: RAW264.7 cell lysate (15 µg/Lane) Lane 9: NIH/3T3 cell lysate (15 µg/Lane) Lane 10: Rat thymus tissue lysate (30 µg/Lane) Predicted band size: 24 kDa Observed band size: 24 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721406) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Flow cytometric analysis of HeLa cells labeling Ran. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721406, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.