Slit2 Recombinant Rabbit Monoclonal Antibody [JE31-87]
cat.: HA721407
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE31-87 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 170 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Slit2 aa 1,380-1,529 / 1,529. |
| Positive control: | HEK-293 cell lysate, 293T cell lysate, PC-3M cell lysate, Raji cell lysate, HCT 116 cell lysate, HeLa cell lysate, rat pancreas tissue lysate, SH-SY5Y cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, A549. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB IF-Cell FC |
1:1,000 1:100 1:50-1:1,000 |
| Uniprot #: | SwissProt: O94813 Human | Q9R1B9 Mouse | Q9WVC1 Rat |
| Alternative names: | Drad 1 E030015M03Rik E130320P19Rik FLJ14420 OTTHUMP00000158695 OTTHUMP00000217852 OTTHUMP00000217853 OTTHUMP00000217854 Slil 3 Slil3 Slit 2 Slit homolog 2 (Drosophila) Slit homolog 2 Slit homolog 2 protein Slit homolog 2 protein C-product Slit-2 Slit2 SLIT2_HUMAN |
Images
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Fig1:
Western blot analysis of Slit2 on different lysates with Rabbit anti-Slit2 antibody (HA721407) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (15 µg/Lane) Lane 2: 293T cell lysate (15 µg/Lane) Lane 3: PC-3M cell lysate (15 µg/Lane) Lane 4: Raji cell lysate (15 µg/Lane) Lane 5: HCT 116 cell lysate (15 µg/Lane) Lane 6: HeLa cell lysate (15 µg/Lane) Lane 7: Rat pancreas tissue lysate (30 µg/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721407) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Slit2 on different lysates with Rabbit anti-Slit2 antibody (HA721407) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane) Lane 2: Human brain tissue lysate (30 µg/Lane) Lane 3: Mouse brain tissue lysate (30 µg/Lane) Lane 4: Rat brain tissue lysate (30 µg/Lane) Predicted band size: 170 kDa Observed band size: 50/200 kDa Exposure time: 28 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721407) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig3:
Immunocytochemistry analysis of A549 cells labeling Slit2 with Rabbit anti-Slit2 antibody (HA721407) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Slit2 antibody (HA721407) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig4:
Flow cytometric analysis of A549 cells labeling Slit2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721407, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.