| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE66-64 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 27 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human HOXC6 aa 1-100 / 235. |
| Positive control: | Raji cell lysate, Daudi cell lysate, HUVEC cell lysate, Jurkat cell lysate, PMVEC cell lysate, rat spinal cord tissue lysate, rat bladder tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P09630 Human Entrez Gene: 252885 Rat |
| Alternative names: | CP25 HHO.C8 Homeo box 3C Homeo box C6 Homeo box C8 protein Homeobox C6 Homeobox protein CP25 Homeobox protein HHO.C8 Homeobox protein Hox-3C Homeobox protein Hox-C6 HOX3 HOX3C Hoxc6 HXC6_HUMAN |
|
Fig1:
Western blot analysis of HOXC6 on different lysates with Rabbit anti-HOXC6 antibody (HA721409) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lane 3: HUVEC cell lysate Lane 4: Jurkat cell lysate Lane 5: PMVEC cell lysate Lane 6: Rat spinal cord tissue lysate Lane 7: Rat bladder tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 1 minute 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721409) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |