HOXC6 Recombinant Rabbit Monoclonal Antibody [JE66-64]
cat.: HA721409
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB
Clonality: Monoclonal
Clone number: JE66-64
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 27 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human HOXC6 aa 1-100 / 235.
Positive control: Raji cell lysate, Daudi cell lysate, HUVEC cell lysate, Jurkat cell lysate, PMVEC cell lysate, rat spinal cord tissue lysate, rat bladder tissue lysate.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P09630 Human
Entrez Gene: 252885 Rat
Alternative names: CP25 HHO.C8 Homeo box 3C Homeo box C6 Homeo box C8 protein Homeobox C6 Homeobox protein CP25 Homeobox protein HHO.C8 Homeobox protein Hox-3C Homeobox protein Hox-C6 HOX3 HOX3C Hoxc6 HXC6_HUMAN
Images
HA721409_1.jpg Fig1: Western blot analysis of HOXC6 on different lysates with Rabbit anti-HOXC6 antibody (HA721409) at 1/1,000 dilution.

Lane 1: Raji cell lysate
Lane 2: Daudi cell lysate
Lane 3: HUVEC cell lysate
Lane 4: Jurkat cell lysate
Lane 5: PMVEC cell lysate
Lane 6: Rat spinal cord tissue lysate
Lane 7: Rat bladder tissue lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 27 kDa
Observed band size: 27 kDa

Exposure time: 1 minute 20 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721409) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.