| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE32-08 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 41 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human HB9/HLXB9/MNX1 aa 276-325 / 401. |
| Positive control: | HeLa cell lysate, Raji cell lysate, MCF7 cell lysate, HepG2 cell lysate, Huh7 cell lysate, PANC-1 cell lysate, SW1990 cell lysate, mouse pancreas tissue lysate. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P50219 Human | Q9QZW9 Mouse | M0R6D8 Rat |
| Alternative names: | HB 9 HB9 HLXB 9 HLXB9 Homeo box HB9 Homeobox HB9 Homeobox protein HB9 HOXHB9 MNX1 MNX1_HUMAN Motor neuron and pancreas homeobox 1 Motor neuron and pancreas homeobox protein 1 Sacral agenesis autosomal dominant (Currarino triad) SCRA 1 SCRA1 |
|
Fig1:
Western blot analysis of HB9/HLXB9/MNX1 on different lysates with Rabbit anti-HB9/HLXB9/MNX1 antibody (HA721410) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: Raji cell lysate (15 µg/Lane) Lane 3: MCF7 cell lysate (15 µg/Lane) Lane 4: HepG2 cell lysate (15 µg/Lane) Lane 5: Huh7 cell lysate (15 µg/Lane) Lane 6: PANC-1 cell lysate (15 µg/Lane) Lane 7: SW1990 cell lysate (15 µg/Lane) Lane 8: Mouse pancreas tissue lysate (30 µg/Lane) Predicted band size: 41 kDa Observed band size: 41 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721410) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |