Lambda Light Chain Recombinant Rabbit Monoclonal Antibody [PD00-56]
cat.: HA721414
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | PD00-56 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 11 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within full length native protein. |
| Positive control: | Human small intestine tissue lysates, human tonsil tissue. |
| Subcellular location: | Secreted, Cell membrane. |
| Recommended Dilutions:
WB IHC-P IF-Tissue |
1:1,000 1:3,000 1:1,000 |
| Uniprot #: | SwissProt: P0CG04 Human | P01701 Human |
| Alternative names: | Constant region of lambda light chains Ig lambda chain C regions IGLC Immunoglobulin lambda constant 1 Mcg marker |
Images
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Fig1:
Western blot analysis of Lambda Light Chain on human small intestine tissue lysates with Rabbit anti-Lambda Light Chain antibody (HA721414) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 11 kDa Observed band size: 25 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721414) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Lambda Light Chain antibody (HA721414) at 1/3,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721414) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
|
Fig3:
Application: IF-Tissue Species: Human Site: tonsil Sample: Paraffin-embedded section Antibody concentration: 1/1,000 |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.