Liver Carboxylesterase 1 Recombinant Rabbit Monoclonal Antibody [JE33-04]
cat.: HA721416
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IF-Cell, FC |
| Clonality: | Monoclonal |
| Clone number: | JE33-04 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 62.5 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Liver Carboxylesterase 1 aa 51-100 / 567. |
| Positive control: | U-937 cell lysate, human liver tissue lysate, human lung tissue lysate, mouse kidney tissue lysate, rat liver tissue lysate, rat kidney tissue lysate, human liver tissue, U-937. |
| Subcellular location: | Endoplasmic reticulum lumen, Cytoplasm, Lipid droplet. |
| Recommended Dilutions:
WB IHC-P IF-Cell FC |
1:1,000 1:1,000 1:100 1:500-1:1,000 |
| Uniprot #: | SwissProt: P23141 Human | Q8VCC2 Mouse | P10959 Rat |
| Alternative names: | ACAT Acyl coenzyme A cholesterol acyltransferase Acyl-coenzyme A:cholesterol acyltransferase Brain carboxylesterase hBr1 Carboxyesterase ES-3 Carboxylesterase Carboxylesterase 1 (monocyte/macrophage serine esterase 1) Carboxylesterase 1 Carboxylesterase 1 deficiency, included Carboxylesterase 2, formerly CE 1 CEH Ces-1 CES1 CES2 CESDD1 Cholesterol ester hydrolase, neutral, macrophage-derived Cholesteryl ester hydrolase Cocaine carboxylesterase EC 3.1.1.1 Egasyn ES-HTEL ES-x Es22 EST1_HUMAN Esterase 22 Esterase hCE 1 HMSE HMSE1 Liver carboxylesterase 1 Liver carboxylesterase 3 Methylumbelliferyl acetate deacetylase 1 MGC117365 MGC156521 Monocyte carboxylesterase deficiency, included Monocyte esterase deficiency, included Monocyte/macrophage serine esterase PCE-1 pI 5.5 esterase Proline-beta-naphthylamidase REH Retinyl ester hydrolase Serine esterase 1 Ses-1 SES1 TGH Triacylglycerol hydrolase |
Images
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Fig1:
Western blot analysis of Liver Carboxylesterase 1 on different lysates with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/1,000 dilution. Lane 1: U-937 cell lysate (20 µg/Lane) Lane 2: Human liver tissue lysate (10 µg/Lane) Lane 3: Human lung tissue lysate (10 µg/Lane) Predicted band size: 62.5 kDa Observed band size: 55 kDa Exposure time: 16 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Western blot analysis of Liver Carboxylesterase 1 on different lysates with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/1,000 dilution. Lane 1: Mouse kidney tissue lysate Lane 2: Rat liver tissue lysate Lane 3: Rat kidney tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 62.5 kDa Observed band size: 55 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721416) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4:
Immunocytochemistry analysis of U-937 cells labeling Liver Carboxylesterase 1 with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. |
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Fig5:
Flow cytometric analysis of U-937 cells labeling Liver Carboxylesterase 1. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721416, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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