ROR2 Recombinant Rabbit Monoclonal Antibody [JE37-72]
cat.: HA721417
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | JE37-72 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 105 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ROR2 aa 34-83 / 943. |
| Positive control: | K-562 cell lysate, U-2 OS cell lysate, HEK-293 cell lysate, Jurkat cell lysate, human placenta tissue. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB IHC-P |
1:1,000 1:1,000 |
| Uniprot #: | SwissProt: Q01974 Human |
| Alternative names: | BDB BDB1 Brachydactyly type B EC 2.7.10.1 MGC163394 Neurotrophic tyrosine kinase Neurotrophic tyrosine kinase, receptor related 2 NTRKR2 Receptor tyrosine kinase-like orphan receptor 2 receptor-related 2 ROR2 ROR2_HUMAN Tyrosine protein kinase transmembrane receptor ROR2 Tyrosine-protein kinase transmembrane receptor Ror2 |
Images
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Fig1:
Western blot analysis of ROR2 on different lysates with Rabbit anti-ROR2 antibody (HA721417) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: U-2 OS cell lysate Lane 3: HEK-293 cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721417) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-ROR2 antibody (HA721417) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721417) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.