Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Human |
Applications: | IHC-P |
Clonality: | Monoclonal |
Clone number: | PD01-27 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 264 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human ROS1 aa 2050-2150. |
Positive control: | Human lung carcinoma tissue. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
IHC-P |
1:200 |
Uniprot #: | SwissProt: P08922 Human |
Alternative names: | c ros 1 c ros c ros oncogene 1 receptor tyrosine kinase c Ros receptor tyrosine kinase c ros1 MCF 3 MCF3 Oncogene ROS OTTHUMP00000017814 OTTHUMP00000017815 OTTHUMP00000040389 Proto oncogene c ros 1 protein Proto oncogene c Ros Proto oncogene tyrosine protein kinase ROS Proto oncogene tyrosine protein kinase ROS precursor Proto-oncogene c-Ros-1 Proto-oncogene tyrosine-protein kinase ROS Receptor tyrosine kinase c ros oncogene 1 Ros 1 ROS 1C ROS ROS proto oncogene 1 , receptor tyrosine kinase ROS_HUMAN ROS1 ROS1C Transmembrane tyrosine specific protein kinase v ros avian UR2 sarcoma virus oncogene homolog 1 v ros UR2 sarcoma virus oncogene homolog 1 |
Fig1:
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Rabbit anti-ROS1 antibody (HA721420) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721420) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |