BACE1 Recombinant Rabbit Monoclonal Antibody [JE30-40]
cat.: HA721421
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE30-40 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 56 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human BACE1 aa 450-501 / 501. |
| Positive control: | Mouse brain tissue lysate, rat brain tissue lysate, human cerebellum tissue lysate, mouse cerebellum tissue lysate. |
| Subcellular location: | Cell membrane, Golgi apparatus, trans-Golgi network, Endoplasmic reticulum, Endosome, Cell surface, Cytoplasmic vesicle membrane, Membrane raft, Lysosome, Late endosome, Early endosome, Recycling endosome, Cell projection, axon, dendrite. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P56817 Human | P56818 Mouse | P56819 Rat |
| Alternative names: | APP beta secretase Asp 2 ASP2 Aspartyl protease 2 BACE 1 BACE BACE1 BACE1_HUMAN Beta secretase 1 Beta secretase Beta site amyloid beta A4 precursor protein cleaving enzyme Beta site amyloid precursor protein cleaving enzyme 1 Beta site amyloid precursor protein cleaving enzyme Beta site APP cleaving enzyme 1 Beta site APP cleaving enzyme Beta-secretase 1 Beta-site amyloid precursor protein cleaving enzyme 1 Beta-site APP cleaving enzyme 1 FLJ90568 HSPC104 Memapsin 2 Memapsin-2 Memapsin2 Membrane associated aspartic protease 2 Membrane-associated aspartic protease 2 Transmembrane aspartic proteinase Asp2 |
Images
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Fig1:
Western blot analysis of BACE1 on different lysates with Rabbit anti-BACE1 antibody (HA721421) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Human cerebellum tissue lysate Lane 4: Mouse cerebellum tissue lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 56 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721421) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.