IGFBP7 Recombinant Rabbit Monoclonal Antibody [JE34-01]
cat.: HA721423
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Monoclonal
Clone number: JE34-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 29 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human IGFBP7 aa 51-250 / 282.
Positive control: HeLa cell lysate, SK-OV-3 cell lysate, SW480 cell lysate, 293T cell lysate, MCF7 cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, rat pancreas tissue, HeLa.
Subcellular location: Secreted.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:1,000
1:1,000
1:100
1:500-1:1,000
Uniprot #: SwissProt: Q16270 Human | Q61581 Mouse
Entrez Gene: 289560 Rat
Alternative names: AGM Angiomodulin FSTL2 IBP-7 IBP7 IBP7_HUMAN IGF binding protein 7 IGF-binding protein 7 IGFBP rP1 IGFBP-7 IGFBP-rP1 IGFBP7 IGFBPRP1 Insulin like growth factor binding protein 7 Insulin-like growth factor-binding protein 7 MAC25 MAC25 protein PGI2 stimulating factor PGI2-stimulating factor Prostacyclin stimulating factor Prostacyclin-stimulating factor PSF RAMSVPS TAF Tumor-derived adhesion factor
Images
HA721423_1.jpg Fig1: Western blot analysis of IGFBP7 on different lysates with Rabbit anti-IGFBP7 antibody (HA721423) at 1/1,000 dilution.

Lane 1: HeLa cell lysate (15 µg/Lane)
Lane 2: SK-OV-3 cell lysate (15 µg/Lane)
Lane 3: SW480 cell lysate (15 µg/Lane)
Lane 4: 293T cell lysate (15 µg/Lane)
Lane 5: MCF7 cell lysate (15 µg/Lane)
Lane 6: Mouse kidney tissue lysate (30 µg/Lane)
Lane 7: Rat kidney tissue lysate (30 µg/Lane)

Predicted band size: 29 kDa
Observed band size: 29 kDa

Exposure time: 43 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721423) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
HA721423_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rabbit anti-IGFBP7 antibody (HA721423) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721423) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
HA721423_3.jpg Fig3: Immunocytochemistry analysis of HeLa cells labeling IGFBP7 with Rabbit anti-IGFBP7 antibody (HA721423) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-IGFBP7 antibody (HA721423) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
HA721423_4.jpg Fig4: Flow cytometric analysis of HeLa cells labeling IGFBP7.

Cells were fixed and permeabilized. Then stained with the primary antibody (HA721423, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.