ODF2 Recombinant Rabbit Monoclonal Antibody [JE35-01]
cat.: HA721425
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE35-01 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 95 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ODF2 aa 780-829 / 829. |
| Positive control: | HEK-293 cell lysate, K-562 cell lysate, MCF7 cell lysate, HeLa cell lysate, U-2 OS cell lysate, Jurkat cell lysate, HepG2 cell lysate. |
| Subcellular location: | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cell projection, cilium, microtubule organizing center, centriole, spindle pole, flagellum. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: Q5BJF6 Human |
| Alternative names: | 84 kDa outer dense fiber protein Cancer/testis antigen 134 Cenexin 1 Cenexin Cenexin1 variant 1 CT134 FLJ44866 KKT 4 KKT4 MGC111096 MGC9034 ODF 2 ODF 84 odf2 ODF2/1 ODF2/2 ODF84 ODFP2_HUMAN OTTHUMP00000022274 OTTHUMP00000022275 Outer dense fiber of sperm tail 2 Outer dense fiber of sperm tails 2 Outer dense fiber of sperm tails protein 2 Outer dense fiber of sperm tails, 84 kD Outer dense fiber protein 2 Sperm outer dense fiber major protein 2 Sperm tail structural protein Testis specific autoantigen |
Images
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Fig1:
Western blot analysis of ODF2 on different lysates with Rabbit anti-ODF2 antibody (HA721425) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: K-562 cell lysate Lane 3: MCF7 cell lysate Lane 4: HeLa cell lysate Lane 5: U-2 OS cell lysate Lane 6: Jurkat cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 95 kDa Observed band size: 95 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721425) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.