PAR2 Recombinant Rabbit Monoclonal Antibody [JE35-05]
cat.: HA721426
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Monoclonal |
| Clone number: | JE35-05 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 44 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human PAR2 aa 328-377 / 397. |
| Positive control: | HCT 116 cell lysate, HepG2 cell lysate, PANC-1 cell lysate, PC-3M cell lysate, AGS cell lysate, SGC-7901 cell lysate, human kidney tissue lysate, human stomach tissue lysate, mouse large intestine tissue lysate, HepG2. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB FC |
1:1,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P55085 Human | P55086 Mouse |
| Alternative names: | Coagulation factor II receptor like 1 Coagulation factor II receptor-like 1 Coagulation factor II thrombin receptor like 1 F2RL1 G protein coupled receptor 11 G-protein coupled receptor 11 GPR11 PAR 2 PAR-2 PAR2_HUMAN Protease activated receptor 2 Proteinase activated receptor 2 Proteinase-activated receptor 2 Thrombin receptor like 1 Thrombin receptor-like 1 |
Images
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Fig1:
Western blot analysis of PAR2 on different lysates with Rabbit anti-PAR2 antibody (HA721426) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: PANC-1 cell lysate (15 µg/Lane) Lane 4: PC-3M cell lysate (15 µg/Lane) Lane 5: AGS cell lysate (15 µg/Lane) Lane 6: SGC-7901 cell lysate (15 µg/Lane) Lane 7: Human kidney tissue lysate (30 µg/Lane) Lane 8: Human stomach tissue lysate (30 µg/Lane) Lane 9: Mouse large intestine tissue lysate (30 µg/Lane) Predicted band size: 44 kDa Observed band size: 55 kDa (Glycosylation) Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721426) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
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Fig2:
Flow cytometric analysis of HepG2 cells labeling PAR2. Cells were fixed and permeabilized. Then stained with the primary antibody (HA721426, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.