TRPC1 Recombinant Rabbit Monoclonal Antibody [JE35-25]
cat.: HA721427
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE35-25 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 91 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TRPC1 aa 408-507 / 793. |
| Positive control: | SH-SY5Y cell lysate, HepG2 cell lysate, A549 cell lysate, MCF7 cell lysate, SK-OV-3 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human brain tissue lysate, mosue brain tissue lysate, mosue testis tissue lysate, rat testis tissue lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P48995 Human | Q61056 Mouse | Q9QX01 Rat |
| Alternative names: | HTRP 1 HTRP1 MGC133334 MGC133335 Mtrp1 Short transient receptor potential channel 1 Transient receptor potential canonical 1 Transient receptor potential cation channel subfamily C member 1 Transient receptor potential channel 1 Transient receptor protein 1 TRP 1 TRP 1 protein Trp related protein 1 TRP-1 TRP1 TRP1 protein TrpC1 Trpc1 transient receptor potential cation channel subfamily C member 1 TRPC1_HUMAN Trrp1 |
Images
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Fig1:
Western blot analysis of TRPC1 on different lysates with Rabbit anti-TRPC1 antibody (HA721427) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate 15 µg/Lane) Lane 2: HepG2 cell lysate 15 µg/Lane) Lane 3: A549 cell lysate 15 µg/Lane) Lane 4: MCF7 cell lysate 15 µg/Lane) Lane 5: SK-OV-3 cell lysate 15 µg/Lane) Lane 6: NIH/3T3 cell lysate 15 µg/Lane) Lane 7: PC-12 cell lysate 15 µg/Lane) Lane 8: Human brain tissue lysate (30 µg/Lane) Lane 9: Mosue brain tissue lysate (30 µg/Lane) Lane 10: Mosue testis tissue lysate (30 µg/Lane) Lane 11: Rat testis tissue lysate (30 µg/Lane) Predicted band size: 91 kDa Observed band size: 120 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721427) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.