TRPC1 Recombinant Rabbit Monoclonal Antibody [JE35-25]
cat.: HA721427
| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE35-25 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 91 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human TRPC1 aa 408-507 / 793. |
| Positive control: | HeLa (Human cervical adenocarcinoma cell) cell lysate, SH-SY5Y (Human neuroblastoma cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB |
1:1,000-1:2,000 |
| Uniprot #: | SwissProt: P48995 Human | Q61056 Mouse | Q9QX01 Rat |
| Alternative names: | HTRP 1 HTRP1 MGC133334 MGC133335 Mtrp1 Short transient receptor potential channel 1 Transient receptor potential canonical 1 Transient receptor potential cation channel subfamily C member 1 Transient receptor potential channel 1 Transient receptor protein 1 TRP 1 TRP 1 protein Trp related protein 1 TRP-1 TRP1 TRP1 protein TrpC1 Trpc1 transient receptor potential cation channel subfamily C member 1 TRPC1_HUMAN Trrp1 |
Images
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Fig1:
Western blot analysis of TRPC1 on different lysates with Rabbit anti-TRPC1 antibody (HA721427) at 1/1,000 dilution. Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate Lane 2: SH-SY5Y (Human neuroblastoma cell) cell lysate Lane 3: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: HA721427, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 91 kDa Observed band size: 120 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.