IGFBP1 Recombinant Rabbit Monoclonal Antibody [JE35-39]
cat.: HA721429
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB
Clonality: Monoclonal
Clone number: JE35-39
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 28 kDa
Isotype: IgG
Immunogen: Recombinant protein within human IGFBP1 aa 1-259/259.
Positive control: Human placenta tissue lysate, human liver tissue lysate.
Subcellular location: Secreted.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P08833 Human
Alternative names: AFBP Alpha pregnancy associated endometrial globulin Amniotic fluid binding protein Binding protein 25 Binding protein 26 Binding protein 28 Growth hormone independent binding protein hIGFBP 1 hIGFBP1 IBP 1 IBP-1 IBP1 IBP1_HUMAN IGF binding protein 1 IGF BP25 IGF-binding protein 1 IGFBP 1 IGFBP-1 IGFBP1 Insulin like growth factor binding protein 1 Insulin-like growth factor-binding protein 1 Placental protein 12 PP 12 PP12
Images
HA721429_1.jpg Fig1: Western blot analysis of IGFBP1 on different lysates with Rabbit anti-IGFBP1 antibody (HA721429) at 1/1,000 dilution.

Lane 1: Human placenta tissue lysate
Lane 2: Human liver tissue lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 28 kDa
Observed band size: 28 kDa

Exposure time: 28 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721429) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.