Ceruloplasmin Recombinant Rabbit Monoclonal Antibody [JE59-01]
cat.: HA721431
Product Type: Recombinant Rabbit monoclonal IgG, primary antibodies
Species reactivity: Rat, Human
Applications: WB
Clonality: Monoclonal
Clone number: JE59-01
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 122 kDa
Isotype: IgG
Immunogen: Synthetic peptide.
Positive control: Rat liver tissue lysate, rat testis tissue lysate.
Subcellular location: Secreted.
Recommended Dilutions:
  WB

1:1,000
Uniprot #: SwissProt: P13635 Rat | P00450 Human
Alternative names: CERU_HUMAN Ceruloplasmin CP 2 CP CP2 Ferroxidase
Images
HA721431_1.jpg Fig1: Western blot analysis of Ceruloplasmin on different lysates with Rabbit anti-Ceruloplasmin antibody (HA721431) at 1/1,000 dilution.

Lane 1: Rat liver tissue lysate
Lane 2: Rat testis tissue lysate

Lysates/proteins at 30 µg/Lane.

Predicted band size: 122 kDa
Observed band size: 140 kDa

Exposure time: 3 minutes 30 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721431) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.