| Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Rat, Human |
| Applications: | WB |
| Clonality: | Monoclonal |
| Clone number: | JE59-01 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 122 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Ceruloplasmin aa 401-450 / 1,065. |
| Positive control: | Rat liver tissue lysate, rat testis tissue lysate. |
| Subcellular location: | Secreted. |
| Recommended Dilutions:
WB |
1:1,000 |
| Uniprot #: | SwissProt: P13635 Rat | P00450 Human |
| Alternative names: | CERU_HUMAN Ceruloplasmin CP 2 CP CP2 Ferroxidase |
|
Fig1:
Western blot analysis of Ceruloplasmin on different lysates with Rabbit anti-Ceruloplasmin antibody (HA721431) at 1/1,000 dilution. Lane 1: Rat liver tissue lysate Lane 2: Rat testis tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 122 kDa Observed band size: 140 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721431) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |