Product Type: | Recombinant Rabbit monoclonal IgG, primary antibodies |
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Species reactivity: | Rat, Human |
Applications: | WB |
Clonality: | Monoclonal |
Clone number: | JE59-01 |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 122 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide. |
Positive control: | Rat liver tissue lysate, rat testis tissue lysate. |
Subcellular location: | Secreted. |
Recommended Dilutions:
WB |
1:1,000 |
Uniprot #: | SwissProt: P13635 Rat | P00450 Human |
Alternative names: | CERU_HUMAN Ceruloplasmin CP 2 CP CP2 Ferroxidase |
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Fig1:
Western blot analysis of Ceruloplasmin on different lysates with Rabbit anti-Ceruloplasmin antibody (HA721431) at 1/1,000 dilution. Lane 1: Rat liver tissue lysate Lane 2: Rat testis tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 122 kDa Observed band size: 140 kDa Exposure time: 3 minutes 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721431) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. |